Assessment of alpha-tocopherol content in cow and goat milk from the Serbian market
2012
Sunarić, S., Faculty of Medicine, Niš (Serbia). Department of Chemistry | Živković, J., Faculty of Medicine, Niš (Serbia). Department of Chemistry | Pavlović, R., Faculty of Medicine, Niš (Serbia). Department of Chemistry | Kocić, G., Faculty of Medicine, Niš (Serbia). Department of Biochemistry | Trutić, N., Faculty of Medicine, Niš (Serbia). Department of Chemistry | Živanović, S., Faculty of Medicine, Niš (Serbia). Laboratory for Chromatography
The present paper reports determination of alpha-tocopherol in commercial cow, raw cow and goat milk, as well as an estimation of its nutritive value based on alpha-tocopherol content. The quantification was done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) and with UV detector set at 286 and 292 nm. The method of milk sample preparation consisted of alkaline saponification with 30% KOH, denaturation of lipoproteins with methanol followed by liquid–liquid extraction with diethyl ether. Recovery values of the extraction method were 78.5, 86.7 and 91.0% for three standard addition levels. Analyzed commercial low-fat milk samples had significant lower alpha-tocopherol levels than milk with higher fat content. Alpha-tocopherol concentrations below 0.30 microg/ml in low-fat cow milk and 0.83–0.86 microg/ml in whole milk were detected. Raw goat milk had much more alpha-tocopherol (1.25 microg/ml) compared with commercial and raw cow milk with similar fat content.
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