Genotyping of bovine viral diarrhea virus in cattle herds by real time qRT-PCR from different regions in Bulgaria and Iraq
2012
Zarkov, I., Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine | Abdulhussein-Jarullah, B., Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine
Bovine viral diarrhea virus (BVDV) as an important member of the Pestivirus genus is a very common agent affecting livestock production throughout the world. Twenty one non vaccinated cattle sera samples collected from different regions of Bulgaria and seventeen from different regions of Iraq were investigated. Samples tested by ELISA antigen capture and cell culture methods were 100% positive by ELISA and cell culture method (some samples propagated after several passage). All isolated viruses were non cytopathogenic on the cells culture, positive results exhibited by direct immunoflourescence stain of virus. Genotyping of all samples obtained from cell culture were tested by Real Time RT-PCR, using Cador BVDV 1/2 kit, after extraction of virus RNA by QIAamp mini kit. All samples were BVDV type 1 only.
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