Polyphenol content and antioxidant activity of commercial blackberry wines from Croatia: Application of multivariate analysis for geographic origin differentiation
2011
Amidžić Klarić, D., University Hospital Dubrava, Zagreb (Croatia) | Klarić, I. | Mornar, A.
Since polyphenolic compounds have been proposed to have beneficial effects on human health as antioxidants, the objectives of this study were to evaluate the polyphenol content and antioxidant activity in 17 commercial blackberry wines (BW) from three different Croatian subregions, and to investigate the relationship between these compounds and geographic origin of BW. A statistically significant difference was found between the concentration of monomeric anthocyanins present in the samples from three subregions. Furthermore, the concentrations obtained for gallic acid were significantly higher than for chlorogenic, caffeic and p-coumaric acids. The half-maximal inhibitory concentrations (IC50) were slightly higher for ABTS (2,2-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) test than for DPPH (2,2-dipheny per l-picrylhydrazyl) test. Based on the results obtained by multivariate data analysis (principal component analysis and linear discriminant analysis) three models were created that gave a satisfactory grouping of the investigated BW based on their geographic origin. The best discriminating model showed not only extreme separation concerning subregions, but also a very narrow area of dispersion of individual wine samples intraregionally.
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