Phytochemical estimation of Sage (Salvia officinalis L.) cultivated in vitro - flavonoids and phenolic acids | Fytochemické hodnotenie šalvie lekárskej (Salvia officinalis L.) kultivovanej in vitro - flavonoidy a kyselina fenolová
2011
Wielgus, K., Institute of Natural Fibres & Medicinal Plants, Poznan (Poland), Department of Biotechnology | Luwanska, A. | Szalata, M. | Mielcarek, S. | Gryszczynska, A. | Lipinski, D. | Zeyland, J. | Szalata, M. | Makowiecka, J. | Mankowska, G. | Slomski, R.
Dried leaves and sage herb (Salviae folium and Salviae herba) present a herbal raw material with an aromatic, camphor scent and a bitter taste. From dried leaves of sage there is a possibility of producing a tincture - Salviae tinctura, with alcohol content from 64 to 69% of ethanol (v/v). In the present work the Bona variety of sage was grown in vitro. Collected leaves were dried and subjected to phytochemical analysis. Axillary and apical buds were used to initiate the in vitro culture. Plant regeneration was carried out on MS medium and BM medium containing the macronutrients according to MS, micronutrients of Nitsch & Nitsch, both supplemented with BAP. The influence of applied medium and explant type on plant regeneration was evaluated by observation of necrosed, contaminated and growing explants. The highest efficiency of regeneration was obtained on MS medium. The phytochemical evaluation of collected leaves showed the content in dry weight of 0.79% flavonoids expressed as hyperosid, 0.57% flavonoids expressed as quercetin and 5.33% polyphenols expressed as rosmarinic acid. The high multiplication rate of plants and the biochemical evaluation of obtained raw material confirmed a possibility of applying sage cultivation by in vitro culture in order to obtain a herbal raw material.
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