Comparison of the influence of selected plant extracts on mitochondria of leukemic cells in vitro
2011
Šutorová, M., University of Veterinary Medicine and Pharmacy in Košice (Slovak Republic) | Tomečková, V. | Mojžišová, G. | Stupák, M.
Individual extracts from horse chestnut, arnica and hops were applied in vitro to leukemic Jurkat cells and the isolated mitochondria from these cells. The influence of plant extracts on mitochondria was observed by means of the determination of the proteins in individual samples and monitoring of the fluorescence of endogenous fluorofores in the control and experimental (exposed to extracts) mitochondria. Fluorofores in nanomolar concentrations were observed by means of the 3 D images of the excitation-emissionmatrix. The resultant fluorescence was manifested graphically as two fluorescent excitation-emission zones. The content of proteins and fluorescence in the first excitation zone was decreased in all experimental mitochondria in comparison with the control. The cytotoxic effects of the tested extracts on mitochondria decreased the most following exposure to arnica and the least after exposure to horse chestnut.
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