The effect of different concentration of trehalose on turkey spermatozoa motility in vitro
2012
Slanina, T., Slovak Univ. of Agriculture in Nitra (Slovak Republic). Faculty of Biotechnology and Food Science, Department of Animal Physiology | Miškeje, M. | Knížat, Ľ. | Mirda, J. | Massányi, P.
Target of this study was to analyze the effect of a different trehalose concentration (T10 – 10 mg per ml; T5 – 5 mg per ml; T2.5 – 2.5 mg per ml; T1.25 – 1.25 mg per ml) on the turkey spermatozoa motility parameters after a culture in vitro at 5 deg C. For control the semen samples were diluted with physiological solution. Individual parameters were recorded at six time periods: 0, 30, 60, 90, 120, 150, 180 minutes. The highest values of spermatozoa motility were determined in samples containing 10 mg per ml of trehalose. The highest motility of 88.16% was recorded after 30 minutes of culture in the sample T10. Significantly (p less than 0.01) higher data were detected in T10 samples compared to control after 90 minutes of culture. After 180 minutes of culture a significantly (p less than 0.01) higher motility was detected in control. In the samples T5; T2.5 and T1.25 similar significantly lower values were recorded compared to control, except for T1.25 at the time of 90 minutes of culture. The highest progressive motility 77.97% was observed in samples T10 after 30 minutes of culture. A significantly lower value in group T10 (p less than 0.05) was detected at time 180. When compared to other control lower values at of all time periods were found.
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