Antibiotics resistance and putative virulence factors of Aeromonas hydrophila isolated from estuary
2012
Odeyemi, O. A., National University of Malaysia, Bangi (Malaysia). School of Biosciences and Biotechnology, Faculty of Science and Technology | Asmat, A. | Usup, G., National University of Malaysia, Bangi (Malaysia). School of Environment and Natural Resources, Faculty of Science and Technology
This study aim to investigate antibiotics resistance profile and putative virulence factors of Aeromonas hydrophila isolated from estuary. Bacteria used for this study were isolated from water and sediment samples obtained from Sungai Melayu, Johor, Malaysia. Serially diluted 100 micro L water and 1g sediment were inoculated on modified Rimler - Shott (mRS) agar. Colonies with distinct cultural characteristics were picked for further studies. Isolates were tested for biofilm productions, protease enzyme and antibiotics resistance profile using agar well diffusion method against 10 commercial antibiotics. Congo Red Agar (CRA), Microplate and Standard Tube (ST) methods were used for assessment of biofilm formation among the isolates while Skim Milk Agar was used for protease production. Sw.KMJ 3 and Sw.KMJ 9 produced black crystalline colonies on CRA. Six of the isolates were biofilm producers in ST method.
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