The effect of deoxynivalenol on rabbit spermatozoa motility in vitro
2012
Medveďová, M., Slovak Univ. of Agriculture in Nitra (Slovak Republic). Faculty of Biotechnology and Food Science, Department of Animal Physiology | Kalafová, A. | Schneidgenová, M. | Maruniaková, N. | Leśniak, A., University of Agriculture, Krakow (Poland). Faculty of Animal Sciences, Department of Animal Physiology and Endocrinology | Latacz, A. | Strzetelska, M. | Capcarová, M. | Bulla, J. | Kolesárová, A.
In this in vitro study the effects of mycotoxin deoxynivalenol (DON) on the motility parameters of rabbit spermatozoa were investigated. The spermatozoa motility was evaluated using CASA assay. Different concentrations of DON in the ejaculate was divided into four experimental group: 0 ng per mL, 10 ng per mL, 100 ng per mL and 1000 ng per mL. Significant differences have not been detected between control group and experimental groups after the detailed analysis of certain motility parameters – total motile spermatozoa (%), progressively motile spermatozoa (%), average path distance (DAP, micro m), average path velocity (VAP, micro m per s) and amplitude of lateral head displacement (ALH, micro m). Observed data suggested that further experiments are needed to be done because of a lack of the evidence toxinogenic effects of mycotoxin DON during its constant detection in feed and food.
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