Determination of natural estrogens in the sediment of coastal area in Japan
2005
Matsuoka, Sumiko | Sakakura, Ran | Takiishi, Mariko | Kurokawa, Yuko | Kawai, Shin'Ichiro | Miyazaki, Nobuyuki
The concentration of natural estrogens such as 17β-estradiol (E2) and estrone (E1) in the sediment of coastal areas in Japan was determined using an enzyme-1inked immunosorbent assay (ELISA). The samples of sediment were collected in Osaka Bay and Otsuchi Bay, Yamada Bay and Ofunato Bay, in Iwate Prefecture, Japan. In addition, core samples were taken in Otsuchi Bay and Yamada Bay, Estrogens in sediments were extracted by sonication in acetone followed by liquid-1iquid extraction with dichloromethane. The levels of E1 were generally higher than those of E2 in sediments, whereas the concentrations of E2 were higher than E1 at some sampling sites. The levels of estrogens in the sites located near estuaries tended to be higher, and levels decreased at the sampling stations nearer to the mouth of the bay However, the estrogen concentration in the sediment collected from near the mouth of bay was highest of all samples in Osaka Bay, both in summer and winter. Core samples were sliced every 2cm from the surface to 20cm deep for the measurement of estrogen. Although the concentrations of estrogens decreased with the depth of core samples, fairly high levels of estrogens were again noticed at the layer deeper than 16cm.
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