Environmental Fate of a Complex Mixture, Creosote, in Two Species of Fish
2006
Sanasack, | Nishimoto, Marc
The metabolic fate of components of creosote, as well as the creosote mixture, was studied in two species of fish, English sole (Pleuronectes vetulus) and rainbow trout (Oncorhynchus mykiss). Laboratory experiments were conducted to assess the metabolism and DNA adduct formation of aromatic compounds by these fish species. These studies were conducted to determine whether the metabolic pathways of creosote components are similar between fish species which have been shown to be susceptible to hepatotoxic effects of components of creosote. In addition, comparisons of the metabolic products of creosote components formed in live animals and by isolated liver cells were made to determine whether isolated hepatocytes may be used as an alternative to live animals in delineating the mechanisms of metabolism of individual compounds and complex mixtures of xenobiotics. Isolated hepatocytes from English sole and rainbow trout were exposed to either benzo(a)pyrene (BaP), a component of creosote, or a creosote extract and the types of metabolites formed were assessed by reversed-phase liquid chromatography (RPLC) or gas chromatography/mass spectrometry (GC/MS). The types of DNA adducts formed during the metabolism of BaP or the creosote mixture were determined using the 32P postlabeling assay. The results showed that BaP was metabolized by English sole and rainbow trout hepatocytes primarily to glucuronide conjugates of hydroxylated BaP derivatives, similar to those detected in bile of English sole exposed to BaP in vivo.
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