Mapping the Key Gene of Fruit Maturity Date in Peach[Prunus persica (L.) Batsch] by SSR markers | 桃果实成熟期主效基因的SSR标记定位
2012
Song Liqin, Hebei Normal University of Science and Technology, Qinhuangdao(China), College of Horticultural Science and Technology | Zhang Libin, Hebei Normal University of Science and Technology, Qinhuangdao(China), College of Horticultural Science and Technology | Zhang Jijun, Hebei Normal University of Science and Technology, Qinhuangdao(China), College of Horticultural Science and Technology
chinois. 桃作为果树中的模式植物，许多重要的质量性状基因已被定位，呈数量性状遗传的果实成熟期是桃的重要经济性状。本研究以大久保桃(Prunus persica (L.) Okubo)自交后代为群体，通过群体分离分析法(bulk segregation analysis, BSA)法对果实成熟期主效基因进行简单重复序列(SSR)标记，在165对SSR引物中筛选出3对与桃果实成熟期相关，分别为：显性标记UDP96-003和共显性标记BPPCT015、UDP97-402。通过对随机群体中的电泳结果进行FsLinkageMap 2.0软件分析和单标记方差分析，得出3个分子标记在一个连锁群上，相对顺序依次为UDP96-003、UDP97-402和BPPCT015，且两两间遗传距离分别为21和13.2 cM，且均与果实成熟期主效基因连锁。用FsQtlMap软件对控制桃果实成熟期基因进行区间定位，得出在BPPCT015和UDP97-402之间存在着一个控制桃果实成熟期的主效数量性状基因座(QTL)，其LOD值为13.35, 效应值达0.623, 对果实成熟期的表型平均遗传值分别为qq:84.59 d、Qq:100.08 d和QQ:115.25 d，即该位点单基因效应值约15 d。该研究结果对前人的推论提供了实验证据，并将该基因锚定在两个SSR标记之间。
Afficher plus [+] Moins [-]anglais. As the model plant in the fruit trees, a number of important quality trait locus has been mapped in peach. Fruit maturity date is an important economic trait in peach. In this study, the self-crossed progenies of peach(Prunus persica (L.) Okubo) were used as material to mapping the maturity genes in peach. 165 SSR primer pairs from the genome of Prunus species were screened by bulked segregant analysis (BSA). Results showed that three primer pairs were related with maturity genes: dominant marker UDP96-003, and co-dominant markers BPPCT015, UDP97-402. The electrophoresis results of these three markers in random samples were analyzed by FsLinkageMap 2.0 and variance analysis for single marker, the result indicated that the rank of the three markers was UDP96-003, UDP97-402 and BPPCT015 with the genetic distance of 21.0 and 13.2 cM, respectively, and all of the three markers were linked with maturity gene. The analysis results from FsQtlMap showed that there was a major-QTL loci between BPPCT015 and UDP97-402 with the LOD of 13.35 and the effect value of 0.623, respectively, and the genetic values were qq :84.59 d, Qq:100.08 d and QQ:115.25 d, respectively. The single-gene effect value of the loci approximately 15 d. The results provide experimental evidence to previous inference, and the locus is anchored between the two SSR markers.
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