Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran
2012
Dariush, S., Guilan Univ., Rasht (Iran Islamic Republic). Dept. of Plant Pathology | Ebadi, A.A., Rice Research Inst. of Iran, Rasht (Iran Islamic Republic) | Khoshkdaman, M., Rice Research Inst. of Iran, Rasht (Iran Islamic Republic) | Rabiei, B., Guilan Univ., Rasht (Iran Islamic Republic). Dept. of Agronomy and Plant Breeding | Elahinia, A., Guilan Univ., Rasht (Iran Islamic Republic). Dept. of Plant Pathology
The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity of 60 strains of the pathogen Pseudomonas syringae pv. syringae obtained from rice and wheat in different growth stages and from different localities in Iran. Cluster analysis by UPGMA method showed that strains were grouped into two clusters. The AMOVA analysis indicated that about 18% of the total genetic variation existed between two populations of rice and wheat, which showed the lack of host specialization in P. s. pv. syringae strains among rice and wheat. We confirmed that high genetic heterogeneity existed in the P. s. pv. syringae strains which are detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be used both to distinguish between strains and to determine relatedness between them.
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