Analysis of Estrogen in Pomegranate Extract by Solid Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry
2010
Kum, E.J., Research Center of Hanil, Anseong, Republic of Korea | Kwon, D.H., Research Center of Hanil, Anseong, Republic of Korea | Shin, H.S., Hankyong National University, Ansung, Republic of Korea
The pomegranate (Punica granatum), especially its fruit, possesses a vast ethnomedical history and represents a phytochemical reservoir of heuristic medical value. The tree and fruit can be divided into several anatomical compartments, and the fruit juice, peel and oil are known to be weakly estrogenic and heuristically of interest for treatment of menopausal symptoms and sequellae. In this study, analysis of estrogen in pomegranate extract was carried out with LC/MS/MS. Three batches of pomegranate extract samples were used to analysis the target compounds (estrogen). The contents of estrogen derivatives in the samples were 38.6 ppb of estriol, 83.5 ppb of estrone, and 10.9 ppb of estradiol. This result suggests that the pomegranate extract can used for treatment of menopause symptoms in the woman.
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