Analysis of Differentially Expressed Genes by Allicin in Human Colorectal Cancer Cell
2010
Kim, K.H., Sungkyunkwan University, Suwon, Republic of Korea | Kim, M.J., Andong National University, Andong, Republic of Korea | Kim, J.S., Andong National University, Andong, Republic of Korea | Pyo, S.N., Sungkyunkwan University, Suwon, Republic of Korea | Kim, B.O., Kyungpook National University, Sangju, Republic of Korea
We investigated anti-cancer and anti-proliferative activity of allicin and analyzed global gene expression changes by allicin treatment in human colorectal HCT116 cells. As a result, allicin decreased cell viabilities in a dose and time-dependent manner and induced apoptosis. Oligo DNA microarray analysis, we found that 7,840 genes were up-regulated more than 2-folds, whereas 10,010 genes were down-regulated more than 2-folds by 50 μM allicin treatment. To confirm specific gene expressions, we performed RT-PCR. Consistent with the results of DNA microarray analysis, allicin dramatically induced ATF3 and NAG1 gene expression. Interestingly, NAG-1 protein expression was dependent on p53 presence. Taken together, our present results increase the knowledge of the molecular mechanism of anti-cancer and anti-proliferative activity mediated by allciin in human colorectal cancer cell.
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