The Dietary Effect of Royal jelly Supplementation for the pre and Post-menopaused Period on Sphingolipid Contents and the Related Enzyme Expression in the Epidermis of Rats
2011
Lee, J.S., Kyung Hee University, Yongin, Republic of Korea | Cho, Y.H., Kyung Hee University, Yongin, Republic of Korea
Epidermal hydration is maintained by epidermal lipid barrier, in which sphingolipids are major lipids. Of various sphingolipids, glucosylceramides (GlcCer) and sphingomyelins (SM) are hydrolyzed to generate various ceramide (Cer) species, of which Cer 1 plays a role in maintaining epidermal barrier. We examined whether dietary supplementation of royal jelly (RJ) during pre and post menopausal period alters epidermal levels of hydration as well as of sphingolipids and their metabolizing enzymes. SD rats were divided into five groups: groups SHAM and OVX (ovariectomy) with control diet, groups RJ1 and RJ2: ovariectomized rats with diet containing 1% 1 or 2 RJ from Korea, group IF: ovariectomized rats with diet containing 80mg/kg isoflavone. Ovariectomy was performed at 4wk of diet supplementation, which was continued for 8 more wks. After 12wks, with no difference of food efficiency, epidermal levels of hydration and total Cer did not differ in all groups. However, despite of higher expression of acid sphing omyelinase (aSMase), an enzyme of SM hydrolysis into Cer, epidermal levels of Cer1, as well as of total GlcCer and SM in group OVX were significantly lowered than in group SHAM, and those of groups RJ2 and IF was similar to in group SHAM. Notably, epidermal levels of GlcCer, SM and Cer1 in group RJ1 were significantly higher than in group OVX, which is paralleled with an increased protein expression of β-glucocerebrosidase (β-GlcCer'ase), an enzyme of GlcCer hydrolysis into Cer. Dietary RJ1 enhances the epidermal levels of GlcCer, SM and Cer1 coupled with an increased protein expression of β-GlcCer'ase.
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