Co-innoculation of rhizobacterial and agrobacterial on growth and root biomass of in vitro banana plantlets.
2008
Zuraida, A.R., MARDI, GPO Box 12301, 50774 Kuala Lumpur (Malaysia). MARDI, GPO Box 12301, 50774 Kuala Lumpur (Malaysia). Bioteknology Research Centre. . | Marziah, M. | Shamsuddin, Z. | Halimi, M.S.(eds.), Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia)
Experiments were carried out to observe the effects of rhizobacterial and agrobacterial coinnoculation on growth and root biomass of in vitro banana plantlets using MS basal medium. Co-innoculation with rhizobacteria (Azospirillum brasilense Sp7, Bacillus sphaericus UPMBIO and Microbacterium oxydens UPMBll) and agrobacterial (Agrobacterium rhizogenes strains AR9402 and A4) showed positive response in enhancing growth and root biomass of banana plantlets where treatment UPMB10+AR9402 was found to be most effective. Combined innoculation has advantages over single imlOculation especially in producing a higher number of roots for the plantlets. The results of the study clearly showed that combined innoculation of rhizobacteria and agrobacteria is a promising technique to enhance the growth of in vitro banana plantlets. Keywords: Rhizobacteria, Agrobacteria, in vitro, banana, PGPR, co-inoculation
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