Cryopreservation of boar semen by pellet freezing
2009
Lamela, D.L., Dr. Emilio B. Espinosa, Sr. Memorial State Coll. of Agriculture and Technology, Mandoon, Masbate (Philippines)
This research was conducted to develop a simple cryopreservation for boar spermatozoa without adverse effects on motility and fertilizing capacity, investigating the optimum glycerol concentration (1, 3, 5, and 7%), equilibration time (15, 30 and 60 min.), and suitable volume (100, 300 and 500 ul) of boar semen for pellet freezing needed for post-thaw motility of boar spermatozoa. Morphological characteristics of the sperm were also considered as a factor affecting sperm motility and fertility. Further investigation was done on the penetrating capability of cryopreserved boar spermatozoa using in vitro matured porcine oocytes. Three percent level of glycerol added to the final dilution equilibrated at either 30 min or 60 min significantly resulted (P0.01) in acceptable percentage of motility, while the three pellet sizes/volumes can be used to cryopreserved boar spermatozoa without adverse effects on its motility. Morphological evaluation of sperms revealed that frozen-thawed semen has much lesser (P0.05) normal sperms than the fresh semen of the same boar. Frozen-thawed semen attained a penetration of 36.15 percent.
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