Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux
2012
Park, S.H., Sogang University, Seoul, Republic of Korea | Lee, S.J., Sogang University, Seoul, Republic of Korea | Kim, B.R., Sogang University, Seoul, Republic of Korea | Lee, S.H., Sogang University, Seoul, Republic of Korea | Lee, J.H., Sogang University, Seoul, Republic of Korea | Sim, S.J., Sungkyunkwan University, Suwon, Republic of Korea | Gu, M.B., Korea University, Seoul, Republic of Korea | Yi, J.H., Seoul National University, Seoul, Republic of Korea | Lee, J.W., Sogang University, Seoul, Republic of Korea
The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO₂) nanoparticles in microorganisms. Nano-sized particles of TiO₂ were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO₂ antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO₂ was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO₂ on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO₂ increased, and glycolysis flux was also higher than the controls.
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