Evaluation of a Fosmid-Clone-Based Microarray for Comparative Analysis of Swine Fecal Metagenomes
2012
Park, S.J., Chungbuk National University, Cheongju, Republic of Korea | Kim, D.H., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Jung, M.Y., Chungbuk National University, Cheongju, Republic of Korea | Kim, S.J., Chungbuk National University, Cheongju, Republic of Korea | Kim, H.I., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Kim, Y.H., Chungbuk National University, Cheongju, Republic of Korea | Chae, J.C., Chonbuk National University, Iksan, Republic of Korea | Rhee, S.K., Chungbuk National University, Cheongju, Republic of Korea
Glass slide arrayed with fosmid clone DNAs generated from swine feces as probes were fabricated and used as a metagenome microarray (MGA). MGA appeared to be specific to their corresponding target genomic fragments. The detection limit was 10 ng of genomic DNA (ca. 10∨6 bacterial cells) in the presence of 1000 ng of background DNA. Linear relationships between the signal intensity and the target DNA (20-100 ng) were observed (r²=0.98). Application of MGA to the comparison of swine fecal metagenomes suggested that the microbial community composition of swine intestine could be dependent on the health state of swine.
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