Selection of microorganism for degradation of oil spoilage
2010
Leesmidt, J. | Sommayo, S. | Sangtanoo, P., Kasetsart Univ. (Thailand). Dept. of Science | Warnpeurch, R. | Vongsthongsri, N. | Amornmongkol, C. | Pongam, S., Ministry of Defense (Thailand). Sattahip and Phangnga Naval Base
Oil contaminated soil and water cause pollution to the environment. Bioremediation/biodegradation by natural microorganisms by which oil pollutants is limited from the environment. This research investigated the efficiency of natural microorganisms for the bioremediation/biodegradation of lubricated oil. Natural microorganisms were collected from oil contaminated sea water and beach sand. Five hundred and nineteen samples were collected from sea coast gulf of Thailand including Chonburi, Rayong, Chanthaburi, Prachuapkhirikhan, Phetchabhuri, Samutsakhon, and Samuthsongkhram. Two hundred and fifty two samples were collected from Andaman sea coast area from 2 provinces including Phangnga and Chumphon. Twenty eight samples were collected from Nakhon pathom, Suphanburi and closed areas, then bacterial isolates were isolated on nutrient agar by spread plate technique founding of pure strains 2,407 bacterial isoaltes. The efficiency of the biodegradation was investigated by natural microorganisms, in qualitative and inoculation on Mineral Salt Medium Agar plates, which composed of 2% (v/v) new lubricated oil and used lubricated oil. The result was found that 60 isolates degraded new lubricated oil and 811 isolates degraded used lubricated oil. These microorganisms were used to quantity test for oil degrading capability on Mineral Salt Medium. The results showed that the 1% (v/v) oil concentration for 96 hours was appropriated for microorganisms. Some species of Bacillus showed the highest degrading 10% (v/v) oil concentration for 96 hours. Future study, the natural microorganisms that had high ability will be used to eliminate oil contaminated soil and water in true conditions.
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