Simultaneous Determination of Luteolin and Luteoloside in Dandelions Using HPLC
2011
Lee, S.L., Chung-Ang University, Anseong, Republic of Korea | Han, S., Chung-Ang University, Anseong, Republic of Korea | Kim, H.M., Chung-Ang University, Anseong, Republic of Korea | Lee, J.M., Chung-Ang University, Anseong, Republic of Korea | Kim, J.K., Chung-Ang University, Anseong, Republic of Korea | Park, C.G., National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea | Lee, S.H., Chung-Ang University, Anseong, Republic of Korea
The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil∨_ 100-5C∧18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mLㆍmin-¹. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817±0.030 mgㆍg-¹) and in T. coreanum leaves (6.205±0.087 mgㆍg-¹), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3-2 times higher than that found in T. ohwianum and T. officinale.
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