Usefulness of Freshwater Alga Water-net (Hydrodictyon reticulatum) as Resources for Production of Fermentable Sugars
2012
Kim, S.K., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Hwang, H.J., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Kim, J.D., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Ko, E.H., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Choi, J.S., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Kim, J.S., Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea
To investigate the usefulness of freshwater alga Water-net (Hydrodictyon reticulatum, HR) as resources for production of fermentable sugars, the easiness of enzymatic saccharification was evaluated at first. When 6 plant materials (HR, Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover) were enzymatically hydrolyzed with 2% solid loading at the same condition, HR showed the highest ratio of saccharification based on glucose production. No milled HR was also completely saccharified at the amounts of optimal enzyme mixture. Glucose yield was not changed though the citrate buffer strength for saccharification was decreased from 0.1 M to 0.1 mM. Only about 10% yield reduction was observed compared to that of 120℃ treatment when HR was enzymatically hydrolyzed at room temperature. The saccharification was normally occurred at 37℃ and pH 6.5 which is general growth condition of fermentable microrganisms, suggesting that HR have a biomass characteristics applicable for the simultaneous saccharification and fermentation. The saccharification was occurred by more than 70~80% of one of the best condition although the supplied enzyme amounts was reduced to 1/10 volume. And the glucose yield by enzymatic hydrolysis was not decreased by 10% HR solid loading and began to decrease at more than 15% solid contents. Above these results show that HR is an interesting algal biomass which is relatively easy to be saccharified by hydrolyzing enzymes. In addition, HR is a flilamentous alga and very easy to be collected. Therefore, HR seems to be an useful and valuable resources in the economical production of fermentable sugars for manufacture of bio-chemical products.
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