Kill curve analysis and response of ethyl methanesulfonate and gamma-rays in diploid and tetraploid cotton
2013
Aslam, U. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics) | Khan, A.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics) | Cheema, H.M.N. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics) | Imtiaz, F. | Malik, W.
Mutagenesis has been used to a good extent to induce genetic variability in plant species to achieve the desired genetic variability. To attain maximum useful mutation density per unit genome and comparative effectiveness of gamma-rays and Ethyl methanesulfonate (EMS), optimal dose for treatment is the key to success. This study focuses on the development of kill curve in three cotton species (Gossypium arboreum, G. barbadense and G. hirsutum). Four genotypes from each of these Gossypium species were treated with eight varying levels of EMS (0.1 to 0.8%) and two doses of gamma-rays (100 to 800 Gy) for kill curve analysis. The data collected on germination percentage, plant height, number of bolls/plant, boll weight, lint yield and its percentage were analyzed and significant interaction among species, genotypes, mutagens and levels of mutagens was observed for all traits under study. Morphologically unusual mutants (rudimentary sparse leaves pattern and broad leaf shape) were also identified from M1 generations of cotton genotypes. Optimal mutagenic doses were calculated based on survival rate and seed viability, which were considered useful in developing cotton mutagenized populations for forward and reverse genetic studies. EMS observed to be more effective than gamma-rays as it generated overall more number of mutants, while later caused higher physical injury in all cotton species.
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