Production and characterization of a novel beta-glucosidase from Fusarium solani
2013
Bhatti, H.N. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry) | Batool, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry) | Afzal, N. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Chemistry)
Beta-glucosidase was produced from Fusarium solani using different agricultural wastes under solid state fermentation (SSF). Optimum production of beta-glucosidase was achieved with rice husk at pH 5, moisture level 60%, and fermentation period of 72 h. Higher yields of beta-glucosidase (3206 U/gds) were obtained from F. solani when the medium was supplemented with lactose. The enzyme was partially purified with ammonium sulfate precipitation yielding a specific activity of 97.5 U/mg. Beta-glucosidase was subjected to various kinetic and thermodynamic parameters to seek the nature of the enzyme. Optimum pH and temperature were found to be 4.5 and 65 degree C, respectively. The enzyme possessed Km 1 mM and Vmax 55.6 mumol min-1. It was also observed that beta-glucosidase was thermally stable up to 65 degree C. Mg2+ ions enhanced the enzyme activity. These characteristics suggest that beta-glucosidase isolated from F. solani could be used for hydrolysis of carbohydrates and cellulosic materials used in textile, paper, starch processing, animal feeds and fruits industries.
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