Fermentacije glicerola pomoću bakterije Streptomyces hygroscopius CH-7 / The fermentation of glycerol by Streptomyces hygroscopius CH-7 bacteria
2012
Ćirić, Jovan T. (Tehnološki fakultet, Leskovac (Srbija)) | Ilić, Slavica B. (Tehnološki fakultet, Leskovac (Srbija)) | Konstantinović, Sandra (Tehnološki fakultet, Leskovac (Srbija)) | Veljković, Vlada B. (Tehnološki fakultet, Leskovac (Srbija)) | Gojgić-Cvijović, Gordana (Institut za hemiju, tehnologiju i metalurgiju, Beograd (Srbija)) | Savić, Dragiša S. (Tehnološki fakultet, Leskovac (Srbija))
Species of the genus Streptomyces produce antibiotics and grow on sub- strates with a different carbon and nitrogen source. The increase of the yield of antibiotics can be achieved at an early stage of the investigation by changing the conditions of fermentation and the composition of the culture medium. During the production process of biodiesel by transesterification of plant oil, except for the target product the raw glycerol in relatively large amounts (10% of the input quantities of oil) is obtained. Glycerol generated in the alkali-cat- alyzed transesterification reaction can be regarded as crude fraction because besides glycerol, there are also traces of soap and dirt from the oil and catalyst residues. The possibility of the production of antibiotics by growing the cultures Streptomyces hygroscopicus CH-7 on the medium containing glycerol as the carbon source was studied in this work. The antibiotics production with crude glycerol remaining from the biodiesel production process from sunflower oil and rape seed oil was monitored under laboratory conditions. The yield of the produced antibiotics hexaene (H-85) and azalomycine and the glycerol con- centration change were followed during the fermentation. The waste glycerol from the biodiesel production from rapeseed oil proved to be the least suit- able carbon source for the cultivation of S. hygroscopicus CH-7 in the antibiot- ics production. Hexaen largest concentration was achived in the medium with waste glycerol from the biodiesel production from sunflower oil (38,7 µg/cm3), more than twice of the amount given to the glucose medium (19,3 µg/cm3). S. hygroscopicus CH-7 produces most azalomycine on the glucose medium, about 25% less on the pure glycerol and much less on the media with crude glycerol.
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