Determination of liposoluble vitamins content in dietetic products
2012
Basić, Zorica N. (Military Medical Academy, Belgrade (Serbia). Institute of Hygiene) | Slavica, Rađen (Military Medical Academy, Belgrade (Serbia). Institute of Hygiene) | Sonja, Radaković (Military Medical Academy, Belgrade (Serbia). Institute of Hygiene)
A large number of dietary products contains high content of vitamins A, E and D. Depending on usage, the amount of consumed vitamins can significantly surpass the recommended daily intake. Also, if the content does not match the declared, this can lead to consumers misleading. In our market, there is a large offer of dietary products in pharmaceutical forms, as well as enriched foods with these vitamins content declared. By proper use of these foods, it is possible to largely meet the daily requirements for vitaminsOur goal was to determine liposoluble vitamin content in products where their content is declared by using HPLC and UPLC methods with spectral and fluorescent detector. For successful determination of the content of vitamins A, E and D in the complex composition of foods multiple purification of samples is necessary, while for the determination of the synthetic products it is enough to apply the extraction. We applied the extraction with n-hexane, extract steaming and reconstitution in methanol, with membrane filtration. Separation was performed with RP-HPLC method with fluorescent detector with variable wavelengths, and UPLC method with the spectral detector. The dietary products of different backgrounds, forms and methods of production (total 148) were analyzed, of which 8.3% had lower, and 2.1% higher content than declared.
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