Setting up a gene expression study for tissue cells by method of quantitative real-time PCR | Nacrtovanje in izvedba studije genskega izrazanja na tkivnih celicah z uporabo metode kvantitativnega PCR v realnem casu
2012
Dusanic, D., Ljubljana Univ. (Slovenia). Biotechnical Fac., Dept. of Animal Science | Bolha, L. | Narat, M. | Oven, I.
Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for casp
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