MUTANT SUNFLOWER LINES, DEVELOPED THROUGH ULTRA SONIC TREATMENT OF IMMATURE EMBRYOS OF GENOTYPE 377 R | МУТАНТНИ ЛИНИИ СЛЪНЧОГЛЕД, СЪЗДАДЕНИ ЧРЕЗ ТРЕТИРАНЕ С УЛТРАЗВУК НА НЕЗРЕЛИ ЗАРОДИШИ ОТ СЛЪНЧОГЛЕД НА ГЕНОТИП 377 R
2013
Encheva, Yu., Dobroudja Agricultural Institute, General Toshevo (Bulgaria)
Immature sunflower zygotic embryos of sunflower fertility restorer line 377 R were treated with ultra sonic before plating to embryo culture medium. Some mutant plants were isolated and self-pollinated for several generations. New sunflower forms with inherited morphological and phytopatological changes were obtained through selection and self-pollination. The genetic changes occurring during the mutation procedure included fourteen morphological characters and mutation for resistance to Septoria helianthi Ellis еt Kellerman. As a result of our investigation, reducing of the mean values were registered for the indices number of branches and length of branches i. e. 14.3 % of the total number of studied characters at line 145 RM. The positive changes were observed for all studied characteristics at line 134 RM, i.e 100 % of traits and for twelve at line 145 RM i.e 86 %. Mutation for resistance to Septoria helianthi Ellis еt Kellerman was obtained from the susceptible Bulgarian fertility restorer line 377 R. The two mutant restorer lines possessed 100 % resistance and stable inheritance in the next generations. Our results showed that mutagenesis in sunflower can be successfully used to develop new lines useful for heterosis breeding.
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