Enzyme activities and motility of boar spermatozoa during 72-hour low temperature storage
2013
Stefanov, R., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Abadzhieva, D., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Chervenkov, M., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Kistanova, E., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Kacheva, D., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Taushanova, P., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Georgiev, B., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria)
The aim of this study was to determine the relationship between semen lactate dehydrogenase isoenzyme X LDH-X and gamma-glutamyl transferase - GGT enzyme activities and sperm motility in boar ejaculates, stored at 15 ºC for 72 h. Ejaculates were obtained from clinically healthy boars from Danube White breed. The motility, concentration and speed parameters of the sperms were determined by sperm class analyzer SCA Microptic, Barcelona, Spain. The measurement of the spermatozoa enzyme activity was made by using a semi-auto clinical chemistry analyzer BA-88 Mindray, Medical Germany GmbH, Bensheim, Germany. It was found that during storage, the decrease in the spermatozoa with progressive motility corresponded to reduced LDH-X and GGT enzyme activities. A stronger correlation was established between LDH-X activity and spermatozoa motility. In conclusion, LDH-X and GGT could be used as biological markers of semen quality after low-temperature 15 ºC storage for seventy two hours.
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