EFFECT OF VITAMIN E AND CYSTEINE ON CRYOPRESERVED BULL SEMEN | ЕФЕКТ НА ВИТАМИН Е И ЦИСТЕИН ВЪРХУ КРИОКОНСЕРВИРАНА СЕМЕННА ТЕЧНОСТ ОТ БИК
2014
Todorova, T., Institute of Animal Science, Kostinbrod (Bulgaria)
Aim of our study was to test the hypothesis that the different levels of vitamin E and cysteine can protect effectively bull sperm against oxidative damage during cryopreservation in various diluents and lead to greater viability and motility of the sperm after thawing. The results show that the lowest amount of malondialdehyde MDA as an indicator of lipid peroxidation - CLP was observed with the addition of 5 mM cysteine to citrate sperm extender. The lowest value of malondialdehyde - MDA as an indicator of lipid peroxidation - CLP was observed after adding 0.1 mM vitamin E to citrate sperm extender. Based on these results we reach the conclusion that cysteine and vitamin E are very effective antioxidants in citrate sperm extender CR and that their addition to such one can reduce oxidative stress caused by thawing and protects bovine sperm by cryopreservation. The addition of cysteine and vitamin E to Tris - egg yolk sperm extender - TP does not improve the quality of the semen. The results of this study provide a new approach to cryopreservation of semen from a bull and can help to improve the results of cryopreservation of semen in industrial farming.
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