Effects of modified 2',5'-oligoadenylates on micropropagation and in vitro and ex vitro rooting of black current (Ribes nigrum) regenerants | Влияние модифицированных 2,5-олигоаденилатов на микроразмножение, укоренение in vitro и ex vitro растений-регенерантов смородины черной (Ribes nigrum)
2012
Kolbanova, E.V. | Kukharchik, N.V., National Academy of Sciences. Scientific and Practical Center for Potato, Vegetable and Fruit Growing (Belarus). Institute for Fruit Growing | Kulak, T.I., National Academy of Sciences, Minsk (Belarus). Institute of Bioorganic Chemistry
The main goal of the research is evaluation of the influence of virazole-containing analogs of 2,5-olygoadenylates on micropropagation and in vitro – ex vitro root formation of black currant (Ribes nigrum) plants during invigoration of the cultivar in vitro. Influence of 2,5-olygoadenylates on vitality level of micro-shoots and propagation coefficient of black currant is analyzed. Root formation of micro-shoots is evaluated. Adaptation of black currant ex vitro on ion-selective substrate is researched. The article demonstrates that 2,5-triadenosinediphosphate derivative contained virazole at the 5'-terminus of oligomer (trimer 1) as well as its 2',5'-anhydro analog (trimer 2) do not adversely effect the vitality and propagation coefficient of black currant cultivars 'Pamyat Vavilova' and 'Klussonovskaya' at the stage of micropropagation. The high concentration of trimmers 1 and 2 (10-6 N) leads to increase of the number of adapted plants, probably due to a high cytokinin activity of these compounds. The treatment with virazole results in lowering number of the survived plants because of phytotoxic effects.
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