Molecular characteristics of the alpha- and beta-tubulin genes of Nosema philosamiae
2013
Zhu, F., Jiangsu Univ. of Science and Technology, Zhenjiang (China) | Shen, Z., Chinese Academy of Agricultural Sciences, Zhenjiang (China). Sericultural Research Insitute | Xu, L., Chinese Academy of Agricultural Sciences, Zhenjiang (China). Sericultural Research Insitute | Guo, X., Chinese Academy of Agricultural Sciences, Zhenjiang (China). Sericultural Research Insitute
Microsporidia are intracellular parasites of insects and other higher eukaryotes. The microsporidian Nosema philosamiae Talukdar, 1961 was isolated from the eri silkworm, Philosamia cynthia ricini Grote. In the present study, alpha- and beta-tubulin genes from N. philosamiae were characterized. The identity analysis of nucleotide and amino acid sequences indicated high similarity with species of Nosema Naegeli, 1857 sensu lato (nucleotide sequences, more or equal than 96.0%; amino acid sequences, more than equal 99.0%). However, the tubulin genes of N. philosamiae share low sequence similarity with that of N. ceranae Fries, Feng, da Silva, Slemenda et Pieniazek, 1996 (strain BRL01) and a Nosema/Vairimorpha species. Phylogenies based on alpha-, beta- and combined alpha- plus beta-tubulin gene sequences showed that N. philosamiae, along with the true Nosema species, forms a separate clade with a high bootstrap value, with N. ceranae BRL01 forming a clade of its own. The results indicated that the alpha- and beta-tubulin sequences may be useful as a diagnostic tool to discriminate the true Nosema group from the Nosema/Vairimorpha group.
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