Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle
2013
Kumar, P., Indian Veterinary Research Institute, Izatnagar (India) | Jangir, B.L., Indian Veterinary Research Institute, Izatnagar (India) | Saikumar, G., Indian Veterinary Research Institute, Izatnagar (India) | Somvanshi, R., Indian Veterinary Research Institute, Izatnagar (India)
The present study was carried out to investigate the involvement of different bovine papillomaviruses in the teat warts of cattle. A total of 11 teat wart samples showing rice grain-like and small, sessile elevated greyish or flesh-like growths were collected from dairy cattle. DNA was extracted from these teat wart samples and PCR and real time PCR techniques were applied using specific primers for BPV-1 and -10 to detect the presence of viral nucleic acid. PCR revealed the presence of viral DNA of BPV-1 and -10 in three and seven samples, respectively. Quantification using real time PCR revealed that the copy numbers of the viral DNA of BPV-1 and -10 DNA varied from 1.12E + 04 to 2.99E + 04 and 3.56E + 02 to 5.23E + 06, respectively. From the present study it can be concluded that BPV-1 and -10 are involved in production of rice grain-like and sessile elevated growths on the teats of cattle.
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