Epidemiological studies on paratuberculosis Johnes disease In livestock
2012
Mohammed, A. M. F.
Out of 144 examined dairy cattle 25 17% showed clinical signs of Johnes disease Mycobacterium avium subsp paratuberculosis MAP ELISA was applied to sera of 143 dairy cattle 34 buffalo 88 sheep and 56 goats with positive percentages of 56% 47% 47% and 38% respectively Standard culture technique was able to detect 11 shedders 23% out of 47 examined cattle MAP DNA was detected in 14 18% out of 76 cattle fecal samples using IS900 PCR MAP was not detected in feces of investigated buffaloes by culture or PCR Duplex F57IC realtime PCR was developed and was found to be more sensitive than IS900 PCR The time to detection of MAP was reduced with increased sensitivity by combination of a pre enrichment step on HEYMMJ for 4 weeks followed by detection of MAP DNA with the developed real time PCR Genotyping using MIRU VNTR typing method revealed slight genetic diversity among 10 Egyptian MAP DNAs and fair one among 17 German MAP isolates with detection of nine patterns using only 3 typing systems Different diagnostics were evaluated compared to culture as gold standard with variable estimates of sensitivity specificity and accuracy There was a poor agreement 0.132 between serum ELISA and IS900 PCR while agreement between milk and serum ELISAs was very good 0.8 There was a slight degree of agreement 0.128 between ASCA and MAP ELISAs as diagnostic tests for Crohns disease which somewhat supports the relationship between MAP and Crohns disease
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