Evaluation of commercial probiotic products on the growth of selected probiotic bacteria strains
2009
Abd El-Ghany, I.H.I. | Mohamed, S.H.S. | Ramadan, F.A.M.
The commercial prebiotic in cereal- based products with bovine colostrum, namely Germa-Fit® and Extra-Fit® as culture media for the growth of selected probiotic lactic acid strains (grown separately): L.gasseria and L.rhamnosus were cultured in MRS (control) and commercial prebiotic products. The media were fermented for 48h at 37°C and analysed for viable cell count and changes in pH values. In Germa-Fit® medium, both probiotic lactic acid strains were attained the highest population (9.6 cfu/ml) after 16 of fermentation time (P≤0.05). With the addition of lactose at 0.5, 1.0 and 1.5% the viability of L.gasseria was improved by approximately 2.1, 2.3 and 1.7 log10 cycles respectively, compared to 3.0, 2.3 and 2.5 for L.rhamnosus after 12h of fermentation time. The effect of Extra-Fit® medium was more positive for growth L.gasseria than that of L.rhamnosus after 8-24h of inoculation time. Generally, Germa-Fit® medium in the presence lactose exhibited the highest growth for both prebiotic strains compared to Extra-Fit® and MRS media. The changes in pH of commercial media were in the optimal pH range (4.5-4.4) for growth of lactic acid bacteria. Therefore, this study suggested that the feasibility use of commercial prebiotic products based- cereal with bovine colostrum in the place the widely used MRS for culture media of probiotic lactic acid bacteria to developing new synbiotic functional fermented dairy food products.
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