Identification and characterisation of host factors involved in plant virus long distance movement
2009
Chapuis , S. (Centre National de la Recherche Scientifique, Strasbourg(France). IBMP Institut de Biologie Moléculaire des Plantes, UPR 2357) | Boissinot , Sylvaine (INRA (France). UMR 1131 Santé de la Vigne et Qualité du Vin) | Brault , Veronique (INRA (France). UMR 1131 Santé de la Vigne et Qualité du Vin) | Revers , Frederic (INRA , Villenave D'Ornon (France). UMR 1090 Génomique, Diversité et Pouvoir Pathogène) | Ziegler-Graff , Véronique (Centre National de la Recherche Scientifique, Strasbourg(France). IBMP Institut de Biologie Moléculaire des Plantes, UPR 2357)
Poleroviruses are an agronomically important genus of plant vlruses (Luteoviridae family) tbat infect a wide range of plants. lnterestingly, tbey are restricted to the phloem tissue and tberefore provide a unique model for studying long distance movement of viruses in plants. Moreover the mechanism underlying this transport, although essential for plant viruses, has never been extensively investigated.The aim of this project is to identify phloem specifie genes, especially those from companion cells that are either deregulated by viral infection or en code proteins that interact directly with viral proteins involved in transport.This project is based on the specifie isolation of companlOn cells by laser capture microdissection that will be used in two different types of experiments:- the construction of a specifie companion cells cDNA library that will serve as a pray in the yeast two hybrid system to screen for proteins interacting with viral factors (bait) involved in long distance movement.- a comparative transcriptional analysis of Arabidopsis thaliana cDNA microarrays using healthy and companion cells infected by BWYV (beet western yellows virus). After hybridization of microarrays, data analysis will supply a set of deregulated phloem genes. Alteration in gene expression will be confirmed and the best candidates identified by quantitative RT-PCR will be further analyzed.The role of a few selected candidate genes identified either by transcriptomic or biochemical (yeast two hybrid) approach will be evaluated regarding virus long distance movement.As this project only recently started, this poster will mainly focus on technical design and optimization of sample preparation and microdissection.
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