Candidate chemosensory genes in the stemborer sesamia nonagrioides
2013
Glaser , Nicolas (INRA , Versailles (France). UMR 1272 Physiologie de l'Insecte : Signalisation et Communication) | Gallot-Le Grand , Aurore (INRA , Versailles (France). UMR 1272 Physiologie de l'Insecte : Signalisation et Communication) | Legeai , Fabrice (Université de Rennes 1, Rennes(France). UMR6074 Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA) - équipe GenScale) | Montagné , Nicolas (INRA , Versailles (France). UMR 1272 Physiologie de l'Insecte : Signalisation et Communication) | Poivet , Erwan (INRA , Versailles (France). UMR 1272 Physiologie de l'Insecte : Signalisation et Communication) | Harry , Myriam (Université Paris 11Centre National de la Recherche Scientifique, OrsayGif-sur-Yvette(France). UPR 9034 Laboratoire Evolution, Génomes et Spéciation) | Calatayud , Paul-André (Institut de Recherche pour le DéveloppementInstitut de Recherche pour le Développement, Gif-sur-YvetteNairobi(France). UR 072 Laboratoire Evolution, Génomes et SpéciationUR 072 ICIPE - NSBB Project) | Jacquin-Joly , Emmanuelle (INRA , Versailles (France). UMR 1272 Physiologie de l'Insecte : Signalisation et Communication)
The stemborer Sesamia nonagrioides is an important pest of maize in the Mediterranean Basin. Like other moths, this noctuid uses its chemosensory system to efficiently interact with its environment. However, very little is known on the molecular mechanisms that underlie chemosensation in this species. Here, we used next-generation sequencing (454 and Illumina) on different tissues from adult and larvae, including chemosensory organs and female ovipositors, to describe the chemosensory transcriptome of S. nonagrioides and identify key molecular components of the pheromone production and detection systems. We identified a total of 68 candidate chemosensory genes in this species, including 31 candidate binding-proteins and 23 chemosensory receptors. In particular, we retrieved the three co-receptors Orco, IR25a and IR8a necessary for chemosensory receptor functioning. Focusing on the pheromonal communication system, we identified a new pheromone-binding protein in this species, four candidate pheromone receptors and 12 carboxylesterases as candidate acetate degrading enzymes. In addition, we identified enzymes putatively involved in S. nonagrioides pheromone biosynthesis, including a Delta 11-desaturase and different acetyltransferases and reductases. RNAseq analyses and RT-PCR were combined to profile gene expression in different tissues. This study constitutes the first large scale description of chemosensory genes in S. nonagrioides.
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