Thr-161 phosphorylation of monomeric Cdc2
2002
de Smedt , Veronique (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Poulhe , Robert (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Cayla , Xavier (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Dessauge , Frederic (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Karaiskou , Anthi (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Jessus , Catherine (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction) | Ozon , Rene (INRA , PARIS (France). USC 0807 USC INRA-Univ. Paris 6 : Physiologie de la reproduction)
Fully grown Xenopus oocyte is arrested at prophase I of meiosis. Re-entry into meiosis depends on the activation of MPF (M-phase promoting factor or cyclin B·Cdc2 complex), triggered by progesterone. The prophase-arrested oocyte contains a store of Cdc2. Most of the protein is present as a monomer whereas a minor fraction, called pre-MPF, is found to be associated with cyclin B. Activation of Cdc2 depends on two key events: cyclin binding and an activating phosphorylation on Thr-161 residue located in the T-loop. To get new insights into the regulation of Thr-161 phosphorylation of Cdc2, monomeric Cdc2 was isolated from prophase oocytes. Based on its activation upon cyclin addition and detection by an antibody directed specifically against Cdc2 phosphorylated on Thr-161, we show for the first time that the prophase oocyte contains a significant amount of monomeric Cdc2 phosphorylated on Thr-161. PP2C, a Mg2+-dependent phosphatase, negatively controls Thr-161 phosphorylation of Cdc2. The unexpected presence of a population of free Cdc2 already phosphorylated on Thr-161 could contribute to the generation of the Cdc2 kinase activity threshold required to initiate MPF amplification.
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