Immunization using GroEL decreases Clostridium difficile intestinal colonization
2013
Pechine , Severine (Université Paris Sud (Paris 11), Châtenay-Malabry(France). Faculté de Pharmacie, Equipe Accueil 4043) | Hennequin , Claire (Université d'Auvergne, Clermont-Ferrand(France). Laboratoire Microorganismes Génome Environnement) | Boursier , Celine (Université Paris Sud (Paris 11), Châtenay-Malabry(France). Faculté de Pharmacie, plate-forme TransProt, Institut Paris-Sud d'innovation thérapeutique) | Hoys , Sandra (Université Paris Sud (Paris 11), Châtenay-Malabry(France). Faculté de Pharmacie, Equipe d’Accueil 4043) | Collignon , Anne (Université Paris Sud (Paris 11), Châtenay-Malabry(France). Faculté de Pharmacie, Equipe d’Accueil 4043)
Clostridium difficile is a pathogen which is responsible for diarrhea and colitis, particularly after treatment with antibiotics. Clinical signs are mainly due to two toxins, TcdA and TcdB. However, the first step of pathogenesis is the colonization process. We evaluated C. difficile surface proteins as vaccine antigens in the hamster model to prevent intestinal colonization. This vaccination induced a partial protection of hamsters against death after a C. difficile challenge. A proteomic analysis of animal sera allowed us to identify proteins which could be responsible for the protection observed. Among these proteins, we identified the GroEL heat shock protein. To confirm the role of the specific GroEL antibodies in the delayed C. difficile colonization of hamsters, we performed an immunization assay in a mouse model. After intranasal immunization with the recombinant protein GroEL, we observed a lower C. difficile intestinal colonization in the immunized group as compared to the control group.
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