La mesure de l'activité de l'eau appliquée au contrôle de l'état hydrique du pollen et des graines d'arbres forestiers
2004
Baldet , Patrick (Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture, Nogent sur Vernisson(France). Domaine des Barres) | Schevin , Patrick (Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture, Nogent sur Vernisson(France). Domaine des Barres) | Vallance , Michel (INRA (France). UR 0588 Unité de recherche Amélioration, Génétique et Physiologie Forestières) | Verger , Michel (INRA (France). UE 0995 Unité Expérimentale d'Amélioration des Arbres Forestiers)
The Water activity R&D project, carried out in cooperation with ONF and Vilmorin, aimed to demonstrate the effectiveness of Water Activity assessment applied to forest seeds and pollen moisture management as a replacement for moisture content assessment. Water Activity assessment is a quick and non destructive technique to qualify the kind and the chemical potential of water remaining in the sample. The growth of micro-organisms require a specific osmotic pressure, or Water Activity(Aw), between cell walls and medium. The reactivity and depreciating ability of hydrolytic reactions, enzymes activity, lipid oxidation or biotic agents growth are dependent on Aw rather than on MC. Consequently, seeds and pollen preservation implies Water Activity control instead of moisture content check. Water dynamics in seeds and pollen have been described by the mean of sorption isotherms, this demonstrates clearly the variability of forest materials and consequently the poor reliability of moisture content for successful moisture management. As a conclusion, Water Activity assessment appears to be a reliable and a quick method to evaluate the ability of a given sample to be stored successfully. This technique has been transferred and is now regularly being used by Vilmorin and ONF for forest seeds and pollen moisture management.
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