Composting bioaerosols : identification of new indicators using molecular tools
2013
Wéry , Nathalie (INRA , Narbonne (France). UR 0050 Laboratoire de Biotechnologie de l'Environnement) | Le Goff , Olivier (INRA , Narbonne (France). UR 0050 Laboratoire de Biotechnologie de l'Environnement) | Bacheley , Hélène (Veolia Environnement , Limay(France). Recherche et Innovation) | Steyer , Jean-Philippe (INRA , Narbonne (France). UR 0050 Laboratoire de Biotechnologie de l'Environnement) | Godon , Jean-Jacques (INRA , Narbonne (France). UR 0050 Laboratoire de Biotechnologie de l'Environnement)
Bioaerosols generated at composting plants are released during processes that involve vigorous movement of material. They are a cause of concern because of their potential impact on the health of workers and residents living close to such facilities. The microbial diversity of bioaerosols collected at five open industrial composting plants treating different types of waste was characterized by a culture-independent approach. Core species of the composting bioaerosols were identified. Among them, three phylotypes were quantified by qPCR in air collected outdoor in natural environments and on ten composting plants together with 4 other microbial markers: cultivable bacteria and fungi, total bacteria (epifluorescence microscopy) and viable bacteria (solid-phase cytometry). A combination of three microbial indicators relevant to monitor composting bioaerosols was defined: one general indicator of bioaerosol emission, the viable bacteria, and two bacterial phylotypes specific to composting bioaerosol, NA07, affiliated to Saccharopolyspora sp. and NC38, affiliated to the Thermoactinomycetaceae. In conclusion, defining the microbial signature of composting aerosols gave us access to indicators that could be used for analyzing bioaerosol dispersal outside composting platforms.
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