Characterization of a novel Xenopus tropicalis cell line as a model for in vitro studies
2012
Sinzelle , Ludivine (Centre National de la Recherche Scientifique, Evry(France). Institute of Systems and Synthetic Biology, Genopole, Universite d’Evry Val d’Essonne, Genavenir 3) | Thuret , Raphaël (University of Manchester, Manchester(Royaume Uni). Faculty of Life Sciences) | Hwang , Ho-Yon (Wellcome Trust Sanger Institute, Hinxton, Cambridge(Royaume Uni). Vertebrate Development and Genetics) | Herszberg , Bérénice (Centre National de la Recherche Scientifique, Evry(France). Institute of Systems and Synthetic Biology, Genopole, Universite d’Evry Val d’Essonne, Genavenir 3) | Paillard , Elodie (Centre National de la Recherche Scientifique, Evry(France). Institute of Systems and Synthetic Biology, Genopole, Universite d’Evry Val d’Essonne, Genavenir 3) | Bronchain , Odile J. (Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique, EvryOrsay(France). Institute of Systems and Synthetic Biology, Genopole, Universite d’Evry Val d’Essonne, Genavenir 3Neurobiologie and Developpement UPR 3294, Institut de Neurobiologie A. Fessard, Universite Paris Sud) | Stemple , Derek L. (Wellcome Trust Sanger Institute, Hinxton, Cambridge(Royaume Uni). Vertebrate Development and Genetics) | Dhorne-Pollet , Sophie (INRA , Jouy-En-Josas (France). UMR 1313 Génétique Animale et Biologie Intégrative) | Pollet , Nicolas(auteur de correspondance) (Centre National de la Recherche Scientifique, Evry(France). Institute of Systems and Synthetic Biology, Genopole, Universite d’Evry Val d’Essonne, Genavenir 3)
Cell lines are useful tools to facilitate in vitro studies of many biological and molecular processes. We describe a new permanent fibroblast-type cell line obtained from disaggregated Xenopus tropicalis limb bud. The cell line population doubling time was _ 24 h. Its karyotype was genetically stable with a chromosome number of 2n 5 21 and a chromosome 10 trisomy. These cells could be readily transfected and expressed transgenes faithfully. We obtained stable transformants using transposon-based gene transfer technology. These cells responded to thyroid hormone and thus can provide a complementary research tool to study thyroid hormone signaling events. In conclusion, this cell line baptized ‘‘Speedy’’ should prove useful to couple in vitro and in vivo biological studies in the X. tropicalis frog model.
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