Expressed sequence tags (ESTs) construction and novel gene mining in Korean native chicken
2004
Shin, S. S. | Shin, J. H. | Han, B. K. | Kim, J. K., | J.Y. Han
We isolated total RNAs and constructed cDNA libraries from four tissues (testis, embryonic gonads, spleen, and brain) of Korean native chicken (KNC). Based on KNC cDNA libraries, total 15,121 clones were sequenced and subsequently assembled into 10,353 contigs. The percentage of singletones in KNC ESTs was 58.9% and approximately 56% of assembled contigs showed the homology to sequences in public databases. Balst analysis showed the homology hits in testis, embrynic gonad, spleen and brain to chicken genes were 6.8%, 7.0%, 14.5%, and 9.2%, respectively. Finally, we predicted the gene functionality and categorized several groups of KNC ESTs according to its functionality.
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