2-Dimensional polyacrylamide gel electrophoresis (2D-PAGE) of papaya ringspot virus (PRSV) infected papaya leaf
2011
Siriwan, W. | Chowpongpang, S., Kasetsart Univ., Kampaengsaen Campus, Nakhom Pathom 73140 (Thailand). Center for Agricultural Biotechnology | Roytrakul, S., National Center for Genetic Engineering and Biotechnology, Science Park Pathumtani 12120 (Thailand). | Takaya, N. | Shimizu, M. | Masuo, S., Tsukuba Univ., Tsukuba, Ibakari (Japan). Graduate School of Life and Environmental Sciences
The Papaya Ringspot virus (PRSV) causes severe economic losses in both papaya and cucurbits throughout the tropics and subtropics regions. PRSV belongs to the potyvirus genus which consists of 30% of known plant virus. The symptoms include mosaic and distortion on papaya leaves, ringspots on fruits, and water soaked-streaks on stems and petioles. The virus stunted papaya plants and drastically reduces the size of fruit. Infected papaya leaf was not confirmed by RT-PCR [reverse transcription-polymerase chain reaction] assay with specific primer for coat protein of PRSV that yielded about 550 bp. Comparison of protein profiles from infected and healthy papaya leaves by Two-dimensional polyacrymlamide gel electrophoresis (2-DE) techniques. Immobilized pH gradient (IPG) strips, pH 4-7 and pH 6-11 were used to analyze the protein extracted from 2 samples of infected and healthy leaves. In this experiment approximately 180 protein spots were detected from the IPG strip of pH 4-7 and 30 protein spots from the 2 samples infected and healthy leaves. Approximately 110 protein spots were differentiated in the IPG strip of pH 6-11 and 20 protein spots in the 2 samples of infected and healthy leaves of papaya plant. The proteins were chosen from different protein spots in 2 different samples and analyzed by MALDI-TOF MS/MS and MASCOT program. Twenty proteins were analyzed by this program. The Lipoxygenase (Lox gene) gene up regulated or elevated contrast healthy plant. An increase in LOX activity in response to infection has been reported for several plant-pathogen systems.
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