Cleaning methods of cattle tripe for consumer safety

At present, Thai people like to consume animal visceral organs (tripe) because tripe is able to bring a variety of cooking, complete nutrition and contains certain minerals than meat. However, tripe from fresh market may be contaminated with chemical residues, non food grade chemical including pathogenic microbes. So, for utilization of by-products from cattle slaughtering process, research aims was to develop a safe process for the utilization of by-product that will benefit consumer and entrepreneurs. Six stomach samples from native cattle were taken for three months (one time per month), washed using different methods and dirt removed. The different methods are as follows: Method 1. (Control group) dipping in hot water (around 70 deg C) approximately one min. Method 2. dipping in cement clear water for 15 min. Method 3. dipping in lime water for 15 min. Method 4. dipping in hot water for about one min. After the sodium hydroxide soak solution provided about 20 mins. then rinse with water (to assist tenderness and bounce or springiness). Method 5. dipping in cement clear water for 15 mins., and dipping in hot water for approximately one min. After the sodium hydroxide soak solution provided about 20 mins then rinse with water. Method 6. dipping in lime water for 15 min. and the stomach was dipped in hot water for approximately one min. After the sodium hydroxide soak solution provided about 20 min. then rinse with water. Method 7. stomach samples from cattle trade market. The samples were analyzed for total microorganisms, including bacteria E.coli, Coliform, Staphylococcus aureus, Salmonella spp. and Clostridium perfingen and analyzed for chemical residues including sodium hydroxide and sulfur dioxide (bleach). The results showed that stomach samples from cattle trade market had the highest chemical residues and E.coli, Coliform, Staphylococcus aureus and Clostridium perfringen. There were no Salmonella spp. Method 1 was a safe process for consumer and tripe was still in natural in color (clear yellow).