Enhanced plantlet regeneration and in vitro root production in vetiver (Vetiveria zizaniodes (L.) Nash)
2012
Cedo, M.L.O. | Licas, M.J.N. | Zara, R.R. | De Guzman, C.C., Philippines Univ. Los Baños, College, Laguna (Philippines). Crop Science Cluster-Crop Physiology Div.
In vitro plantlet regeneration and production of roots in the shoot base explants of vetiver (Vetiveria zizanioides (L.) Nash was investigated using Murashige and Skoog's (MS) medium supplemented with different types and concentrations of plant growth regulators. Extensive shoot proliferation was maintained at 5 mg/L 6-benzyl-aminopurine (BAP). Increasing the concentration of BAP to 20 mg/L significantly inhibited the growth of shoots. Root production was greatly enhanced by the addition of either 0.25 mg/L naphthalene acetic acid (NAA) or 20 mg/L indole-3-butyric acid (IBA). Roots induced by NAA were short and thick, whereas those induced by IBA were long, thin and fibrous. Supplementation of 0.5 mg/L NAA to MS basal medium and further enrichment with 0.5 mg/L kinetin (ki) significantly enhanced the proliferation of roots. However, addition of 0.2 mg/L paclobutrazol (PBZ) into the NAA-supplemented MS medium suppressed both shoot and root growth of vetiver.
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