Molecular investigations to determine the ectomycorrhizal habit of Lactarius sanguifluus associated with coniferous and deciduous vegetation of Galyat, Khyber Pakhtunkhwa, Pakistan

Ilyas, S. . Dept. of Botany; Razaq, A. . Dept. of Botany; Khalid, A.N. . Dept. of Botany

Molecular investigations to determine the ectomycorrhizal habit of Lactarius sanguifluus associated with coniferous and deciduous vegetation of Galyat, Khyber Pakhtunkhwa, Pakistan

2013

Ilyas, S. (Punjab Univ., Lahore (Pakistan). Dept. of Botany) | Razaq, A. (Punjab Univ., Lahore (Pakistan). Dept. of Botany) | Khalid, A.N. (Punjab Univ., Lahore (Pakistan). Dept. of Botany)

This is the first detailed report on molecular identification of aboveground fruiting body as well as its ectomycorrhiza, Lactarius species, associated with wide host range. Lactarius sanguifluus growing in association with different host trees: Juglans, Populus and Quercus were collected from Galyat, Pakistan along with soil blocks containing plant roots. The genomic DNA was extracted from basidiocarps and roots of different trees. The internal transcribed spacers (ITS) region of rDNA was amplified using forward primer ITS1F and reverse primer ITS4, which generated fragments of 700-750bp. The aboveground sequences showed base similarity more than 99% with L. sanguifluus submitted from Europe (FJ858746, AF249289). The phylogenetic analysis of above and belowground parts of L. sanguifluus showed their clustering in same clade with same species. Sequence data of seven specimens is being published from Pakistan; two belong to epigeous L. sanguifluus (HF559378.1, HF559379.1), four ectomycorrhizal roots; two associated with Quercus incana (HF559374.1, HF559375.1), one associated with Pinus wallichiana (HF559376.1), one associated with Juglans regia (HE615155) and one ectomycorrhizal sequence of L. semisanguifluus (HF559377.1) associated with Q. incana.

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