In vitro induction of mutation in tomato (Lycopersicon esculentum L.) cv. Roma by using chemical mutagens
2012
Ishfaq, M. (Punjab Univ., Lahore (Pakistan). Dept. of Botany) | Nasir, I.A. (Punjab Univ., Lahore (Pakistan). National Centre of Excellence in Molecular Biology) | Mahmood, N. (Punjab Univ., Lahore (Pakistan). Inst. of Mycology and Plant Pathology) | Saleem, M.
The aim of present study was to optimize tissue culture conditions for tomato (Lycopersicon esculentum L.) cv. Roma to induce mutagenesis with different chemical mutagens (Cholchicine, Sodium azide, and Methyl-N-Nitro-N-Nitrosoguanidine abbreviated as MNNG). Tomato seeds were surface sterilized with 0.05 % HgCl2 and placed on the Petri plates padded with wet cotton, obtained plantlets in 6-7 days. Meristem was isolated from the germinated plants to get pathogen free plants. Media with different hormone compositions were used for callus induction and regeneration. Media having BAP (2.22 muM) proved to be the best callus induction media, while media having IAA (0.75 muM) and zeatin (9.12 muM) was found to be best for regeneration. The calli were treated with different concentrations (0.1 mM- 0.5mM) of the mutagens colchicine, sodium azide and MNNG for ten days. Alter mutagenic treatment. calli were transferred in regeneration medium with said concentration of hormones and growth parameters of the mutated plants were recorded in comparison to control plants.
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