Role of fluorescent Pseudomonas associated with root nodules of soybean in suppressing the root rotting fungi and root knot nematode of soybean in soil amended with seeds of Vernonia antihelmenthica

Batool, H. . Dept. of Botany; Fatima, N. . Dept. of Botany; Hira, K. . Dept. of Biochemistry; Sultana, V. . Dept. of Biochemistry; Ara, J. . Dept. of Food Science and Technology; Ehteshamul-Haque, S.

Role of fluorescent Pseudomonas associated with root nodules of soybean in suppressing the root rotting fungi and root knot nematode of soybean in soil amended with seeds of Vernonia antihelmenthica

Batool, H. (Karachi Univ. (Pakistan). Dept. of Botany) | Fatima, N. (Karachi Univ. (Pakistan). Dept. of Botany) | Hira, K. (Karachi Univ. (Pakistan). Dept. of Biochemistry) | Sultana, V. (Karachi Univ. (Pakistan). Dept. of Biochemistry) | Ara, J. (Karachi Univ. (Pakistan). Dept. of Food Science and Technology) | Ehteshamul-Haque, S.

Soilborne plant pathogens, root rotting fungi and root knot nematodes are major yield limiting factors in the production of food, fiber and ornamental crops. In this study, Pseudomonas aeruginosa (PGPR-104) and Bradyrhizobium japonicum (NFB-49) isolated from the root nodule of cultivated soybean plant (Glycine max (Merr) and examined for their nematicidal and fungicidal activity. In vitro studies of cell free culture filtrates of PGPR-104 and NFB-49 showed nematicidal activity against illleloidogyne javanica. The strains PGPR-104 and NFB-49 showed more than 90% mortality. Antifungal activity of PGPR strains were tested against three common and destructive plant pathogenic fungi viz., Macrophomina phaseolina, Rhizoctonia solani and Fusarium solani. Both the bacterial strains, PGPR-104 and NFB-49 significantly inhibited the growth of pathogenic fungi. The plant protection potential of theses strains were evaluated in screen house on soybean in soil amended with Vernonia antihelmenthica seeds and compared with biocontrol isolate of P. aeruginosa and B. japonicum, fungicides topsin-M and nematicide carbofuran. Biocontrol agents (PGPR, rhizobia) and organic amendment (V. antihelmenthica seeds powder) significantly (p less than 0.001) control the infection caused by root rotting fungi M. phaseolina, R. solani and F. solani and root knot nematodes M. javanica on soybean. Endo-nodule PGPR, rhizobia and Vernonia soil amendment significantly improved soybean growth by producing taller plants and greater fresh shoot weight.

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