Virus induced gene silencing of SLP4H3 and SLP4H8 induces alterations in plant growth
Akcesme, F. B.
Prolyl 4-hydroxylases (P4Hs) belong to the family of 2-oxoglutarate-dependent dioxygenases and catalyze the formation of 4-hydroxyproline, requiring 2-oxoglutarate and O2 as co-substrates and iron(II) ion as a co-factor. Nine putative P4Hs have been identified up to now in the tomato genome. A reverse genetics approach, Virus-Induced Gene Silencing, was used to silence two P4Hs, P4H3 and P4H8. Transient silencing of P4H3 and P4H8 altered tomato plant growth. P4H3- and P4H8-VIGS plants had shorter stems, reduced fresh weight and smaller leaves, due to suppressed cell expansion of leaf epidermal cells. These results indicate that P4H3 and P4H8 play a significant role in tomato plant growth and VIGS is a useful tool to study the function of tomato gene families such as P4Hs in growth and development, and elucidate, in the long run, the physiological significance of substrate-proteins such as hydroxyproline-rich glycoproteins.
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